Identification of lipopeptide xantholysins from <i>Pseudomonas</i> sp. DJ15 and their insecticidal activity against <scp><i>Myzus persicae</i></scp>

Abstract

Abstract Myzus persicae is an important insect pest that reduces crop production worldwide. The use of pesticides for aphid control has generated much concern related to insect resistance and undesirable environmental effects. In an effort to discover new alternatives to counter M. persicae , we found that Pseudomonas isolate DJ15 produced insecticidal metabolites. To isolate the insecticidal metabolites, a cell‐free supernatant of DJ15 was extracted and subjected to bioassay‐guided chromatography. Based on the structures elucidated in instrumental analyses, the metabolites were identified as xantholysins A and B. The metabolites showed strong insecticidal activity against M. persicae with 50% mortality at levels of 13.4 and 24.6 μg/mL for xantholysins A and B, respectively. This is the first study to identify xantholysins as insecticidal metabolites against M. persicae .