Lymphocyte chemotaxis in inflammation. X. Heterogeneity of chemotactic responsiveness in human T subsets towards lymphocyte chemotactic factors from delayed hypersensitivity reaction site.

Abstract

In previous studies, four lymphocyte chemotactic factors (LCF-a, -b, -c and -d) were isolated from delayed-type hypersensitivity (DTH) skin reaction sites of guinea-pig. In tests on guinea-pig lymphocytes, LCF-a attracted B cells, whereas LCF-b, -c and -d attracted T cells. We now report the chemotactic responses of human lymphocytes to the guinea-pig LCFs. LCF-a strongly attracted B lymphocytes and weakly attracted T lymphocytes, whereas LCF-b, -c and -d predominantly attracted T lymphocytes. In tests on T-lymphocyte subsets, LCF-b and -d selectively attracted OKT4 subsets and theophylline-resistant T cells with helper phenotype. In contrast, LCF-c attracted OKT4 and OKT8 subsets, and both theophylline-resistant and sensitive subsets (i.e. cells with both helper and suppressor phenotype). The results indicate that the different T-cell subsets migrate selectively into sites of inflammation.