In vitro oxidative decarboxylation of free fatty acids to terminal alkenes by two new P450 peroxygenases

Abstract

Our data identify CYP-Sm46Δ29 as an efficient OleT_JE-like fatty acid decarboxylase. Oxidative decarboxylation chemoselectivity of the CYP152 decarboxylases is largely dependent upon the carbon chain length of fatty acid substrates and their precise positioning in the enzyme active site. Finally, the kinetic mode analysis of the enzymes could provide important guidance for future process design.