Angewandte Chemie International Edition · 2017 · 108 citations · 28 references
Life SciencesBiochemistryCell BiologyBiosensorsMolecular ImagingRedox BiochemistryAntioxidant DefenseFluorescence SpectroscopyOxidative Damage To BiomoleculesOxidative StressReactive Oxygen SpeciesReactive Oxygen Species BiologyFluorescence Imaging ProbesFluorescent ProbesFluorescent Reactive Oxygen Species ProbesHydroxyl Radical GenerationOxidative Stress DetectionRadical ScavengersReactivitySensitivity
Abstract The hydroxyl radical ( . OH), one of the most reactive and deleterious reactive oxygen species (ROS), has been suggested to play an essential role in many physiological and pathological scenarios. However, a reliable and robust method to detect endogenous . OH is currently lacking owing to its extremely high reactivity and short lifetime. Herein we report a fluorescent probe HKOH‐1 with superior in vitro selectivity and sensitivity towards . OH. With this probe, we have calibrated and quantified the scavenging capacities of a wide range of reported . OH scavengers. Furthermore, HKOH‐1r, which was designed for better cellular uptake and retention, has performed robustly in detection of endogenous . OH generation by both confocal imaging and flow cytometry. Furthermore, this probe has been applied to monitor . OH generation in HeLa cells in response to UV light irradiation. Therefore, HKOH‐1 could be used for elucidating . OH related biological functions.
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A Common Mechanism of Cellular Death Induced by Bactericidal Antibiotics
Michael A. Kohanski, Daniel J. Dwyer, Boris Hayete et al. · Cell · 2007 · 3K citations · Full text
Bhavani Balasubramanian, Wendy Pogozelski, Thomas D. Tullius · Proceedings of the National Academy of Sciences · 1998 · 556 citations