Antibacterial activity of nanoparticles from Allium sp

Abstract

The plant material such as garlic and onion used in this study was collected from the nearby grocery, Thingal Nager, Kanyakumari District. The pathogens used in this study such as E.coli, Proteus sp., Klebsiella sp., Staphylococcus sp., Enterobacter sp., Bacillus sp. and Pseudomonas sp. were collected from Vivek Laboratory, Nagercoil. The plant material was ground in blender and extracted with distilled water. The plant extract were added with 3-10mm AgNO3 and incubated for the synthesis of plant silver nano particles and it was confirmed by UV-visible spectroscopy. After that the antimicrobial activity was determined by disc diffusion method. Antibacterial disc were prepared in 5 different concentrations such as 100μl, 200μl, 300μl, 400μl, 500μl respectively. The antimicrobial activity of particles from garlic (16.65±0.10) showed greater activity than particles from onion (12.93±0.15). This study showed that the extract reveals the concentration dependent activity. The study revealed that the silver nano particles from plant extracts could be as a therapeutic agent for human microbial infections. Key words: silver nano particles, pathogens, garlic, onion, antimicrobial activity.