Kinetic Studies on Microbial Activities in Concentrated Solutions.

Abstract

Decreases of respiration rates in the presence of excess sugars, substrates and non-substrates, were investigated kinetically on the cell free preparation of a Pseudomonas. The schemes of enzyme inhibition which include successive inactive complexes of one mole of enzyme and two and three moles of substrate or one mole of enzyme and one and two moles of inhibitor were shown to fit the experimental results. Water concentrations were not enough to explain the different inhibitory properties of sugars.