Efficient Antibody Assembly in E. coli Periplasm by Disulfide Bond Folding Factor Co-expression and Culture Optimization

A novel coumarin‐labelled peptide for sensitive continuous assays of the matrix metalloproteinases

Christopher G. Knight, Frances Willenbrock, Gillian Murphy · FEBS Letters · 1992 · 707 citations

Life Sciences, Biochemistry, Pharmacology +12

Overlap Extension PCR Cloning: A Simple and Reliable Way to Create Recombinant Plasmids

Anton V. Bryksin, Ichiro Matsumura · BioTechniques · 2010 · 641 citations · Full text

Life Sciences, Biotechnology, Molecular Biology +17

Efficient folding of proteins with multiple disulfide bonds in the <i>Escherichia coli</i> cytoplasm

Paul Bessette, Fredrik Åslund, Jon Beckwith et al. · Proceedings of the National Academy of Sciences · 1999 · 634 citations · Full text

Life Sciences, Biochemistry, Molecular Biology +17

Defining the roles of the periplasmic chaperones SurA, Skp, and DegP in <i>Escherichia coli</i>

Joseph G. Sklar, Tao Wu, Daniel Kahne et al. · Genes & Development · 2007 · 470 citations · Full text

Life Sciences, Microbiology, Molecular Biology +16

Strategies for successful recombinant expression of disulfide bond-dependent proteins in Escherichia coli

Ario de Marco · Microbial Cell Factories · 2009 · 364 citations · Full text

Life Sciences, Biochemistry, Microbiology +15