Bratislavské lekárske listy/Bratislava medical journal · 2017 · 10 citations · 7 references
PharmacyPharmacologyToxicologyDna RepairGenetic ToxicologyMechanisms Of ToxicityCytotoxicity AssessmentCytotoxicity EvaluationDna DamageGenotoxicity AssessmentGenotoxicity MechanismsGenotoxicity TestingDrug-induced Dna DamageGenotoxic EndpointsGenotoxicityGenotoxicity ReductionLymphocyte Genotoxic DamageMitotic FiguresPeripheral Blood Lymphocyte AnalysisSister Chromatid Exchange Analysis
OBJECTIVE: Protective effect of thymoquinone (TQ) against the cytotoxic and genotoxic effects of cyclophosphamide (CP) was assessed in human peripheral blood lymphocyte culture. METHODS: Mitotic indices were determined as endpoints of cytotoxicity, while sister chromatid exchanges (SCE) served as endpoints of genotoxicity. Firstly, the genotoxic effect of 0.16 µg/ml of CP was tested and CP was detected as genotoxic. In the second set, CP group was treated with 20 µM and 40 µM TQ. RESULTS: TQ reduced the SCE frequencies, suggesting its protective action on human lymphocytes in vitro against the CP induced genotoxic damage. CONCLUSIONS: Our results suggest that TQ produces a protective mechanism against CP-induced genetic damage, and suggest a role of DNA strand breaks in the genotoxicity (Tab. 1, Fig. 1, Ref. 19).
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Thymoquinone Induces Telomere Shortening, DNA Damage and Apoptosis in Human Glioblastoma Cells
Resham L Gurung, Shi Ni Lim, Aik Kia Khaw et al. · PLoS ONE · 2010 · 175 citations · Full text
Medicine, Oncology, Pharmacology +17
Clinical Pharmacology of Cyclophosphamide and Ifosfamide
Jing Zhang, Quan Tian, Shu‐Feng Zhou · Current Drug Therapy · 2006 · 113 citations
Ahmet Dirican, Harika Atmaca, Emir Bozkurt et al. · Clinical & Translational Oncology · 2014 · 81 citations
Medicine, Oncology, Pharmacology +17