Concepedia

Synthesis of new hydrophilic rhodamine based enzymatic substrates compatible with droplet-based microfluidic assays

Johan Fenneteau, Dany Chauvin, Andrew D. Griffiths, Clément Nizak, Janine Cossy

Chemical Communications · 2017 · 26 citations · 30 references

DOI

Concepts

Abstract

Here we report the conception, synthesis and evaluation of new hydrophilic rhodamine-based enzymatic substrates for detection of peptidase activity compatible with high-throughput screening using droplet-based microfluidics.

References

30

Relative fluorescence quantum yields using a computer-controlled luminescence spectrometer

Alun T. Rhys Williams, S.A. Winfield, James N. Miller · The Analyst · 1983 · 1.4K citations

, , +8

Ultrahigh-throughput screening in drop-based microfluidics for directed evolution

Jeremy J. Agresti, Eugene Antipov, Adam R. Abate et al. · Proceedings of the National Academy of Sciences · 2010 · 1.2K citations · Full text

, , +17

Emergence of a catalytic tetrad during evolution of a highly active artificial aldolase

Richard Obexer, Xavier Garrabou, Peer R. E. Mittl et al. · Nature Chemistry · 2016 · 319 citations

, , +14

A completely in vitro ultrahigh-throughput droplet-based microfluidic screening system for protein engineering and directed evolution

Ali Fallah-Araghi, Jean‐Christophe Baret, Michaël Ryckelynck et al. · Lab on a Chip · 2012 · 261 citations

, , +16

Controlling molecular transport in minimal emulsions

Philipp Gruner, Birte Riechers, B. Semin et al. · Nature Communications · 2016 · 226 citations · Full text

, , +17