Ephrin-B2/Fc promotes proliferation and migration, and suppresses apoptosis in human umbilical vein endothelial cells

Abstract

// Li-Chun Zheng 1, 2, * , Xiao-Qing Wang 2, * , Kun Lu 3, * , Xiao-Ling Deng 3 , Cheng-Wei Zhang 4 , Hong Luo 2 , Xu-Dong Xu 2 , Xiao-Man Chen 2 , Lu Yan 5 , Yi-Qing Wang 2 and Song-Lin Shi 3 1 Medical College of Xiamen University, Jinshan Community Health Service Center, Xiamen Traditional Chinese Medical Hospital, Xiamen 361000, P.R. China 2 Xiamen Heart Center, Medical College of Xiamen University, Xiamen 361000, P.R. China 3 Department of Basic Medicine, Medical College of Xiamen University, Cancer Research Center of Xiamen University, Xiamen 361102, P.R. China 4 Department of Cardiology, Affiliated Dongnan Hospital of Xiamen University, Zhangzhou 363000, P.R. China 5 Department of Basic Medicine, Medical College of Xiamen University, Xiamen 361102, P.R. China * These authors have contributed equally to this work Correspondence to: Yi-Qing Wang, email: wang_gina@163.com Song-Lin Shi, email: shisonglin@xmu.edu.cn Keywords: ephrin-B2, proliferation, migration, human umbilical vein endothelial cell, proteomic analysis Received: December 09, 2016     Accepted: April 03, 2017     Published: April 20, 2017 ABSTRACT Tumor growth and metastasis are angiogenesis dependent. Angiogenic growth involves endothelial cell proliferation, migration, and invasion. Ephrin-B2 is a ligand for Eph receptor tyrosine kinases and is an important mediator in vascular endothelial growth factor-mediated angiogenesis. However, research offer controversial information regarding effects of ephrin-B2 on vascular endothelial cells. In this paper, proteome analyses showed that ephrin-B2/Fc significantly activates multiple signaling pathways related to cell proliferation, survival, and migration and suppresses apoptosis and cell death. Cytological experiments further confirm that ephrin-B2/Fc stimulates endothelial cell proliferation, triggers dose-dependent migration, and suppresses cell apoptosis. Results demonstrate that soluble dose-dependent ephrinB2 can promote proliferation and migration and inhibit apoptosis of human umbilical vein endothelial cells. These results also suggest that ephrinB2 prevents ischemic disease and can potentially be a new therapeutic target for treating angiogenesis-related diseases and tumors.