Isoxazole derivatives as new nitric oxide elicitors in plants

Abstract

Several 3,5-disubstituted isoxazoles were obtained in good yields by regiospecific 1,3-dipolar cycloaddition reactions between aromatic nitrile oxides, generated in situ from the corresponding hydroxyimidoyl chlorides, with non-symmetrical activated alkynes in the presence of catalytic amounts of copper(I) iodide. Effects of 3,5-disubstituted isoxazoles on nitric oxide and reactive oxygen species generation in <i>Arabidopsis</i> tissues was studied using specific diaminofluoresceine dyes as fluorescence indicators.