Concepedia
Applied Microbiology and Biotechnology · 2017 · 18 citations · 25 references
Life SciencesBiotechnologyMicrobiologyBacteriologyMicrobial BiotechnologyMicrobial GeneticsBiocatalyst ScreeningEnzyme ScreeningBlue-white ScreeningColorimetric ScreeningEscherichia Coli
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One-step inactivation of chromosomal genes in <i>Escherichia coli</i> K-12 using PCR products
Kirill A. Datsenko, Barry L. Wanner · Proceedings of the National Academy of Sciences · 2000 · 15.2K citations · Full text
Life Sciences, Genetics, Molecular Genetics +17
Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors
Celeste Yanisch-Perron, Jeffrey Vieira, Joachim Messing · Gene · 1985 · 15.2K citations
Life Sciences, Microbiology, Molecular Biology +11
Automated design of synthetic ribosome binding sites to control protein expression
Howard M. Salis, Ethan A. Mirsky, Christopher A. Voigt · Nature Biotechnology · 2009 · 1.9K citations · Full text
Life Sciences, Biotechnology, Molecular Biology +16
Characterization of Sfp, a <i>Bacillus subtilis</i> Phosphopantetheinyl Transferase for Peptidyl Carrier Protein Domains in Peptide Synthetases
Luis E. N. Quadri, Paul H. Weinreb, Ming Lei et al. · Biochemistry · 1998 · 701 citations
Life Sciences, Biochemistry, Molecular Biology +12
Golden Gate Cloning
Carola Engler, Sylvestre Marillonnet · Methods in molecular biology · 2013 · 250 citations