Spectrophotometric determination of Pregabalin using 1, 2-Napthaquinone-4- sulfonic acid Sodium and 2, 4 dinitrophenyl hydrazine in pharmaceutical dosage form.

Abstract

Two simple, extraction free spectrophotometric methods (Method-1 and 2) for the quantitative estimation of pregabalin (PGB) in bulk drugs and pharmaceutical formulations (Capsules) have been developed. The first method is based on the condensation of pregabalin with 1, 2- napthaquinone-4- sulfonic acid sodium (NQS) in alkaline media to yield orange colored products. Pregabalin at its λmax 485 nm shows linearity in the concentration range of 5- 45μg/ml. The first method is based on the oxidation of 2, 4- dinitrophenylhydrazine (2, 4-DNP) and coupling of the oxidized product with drugs to give intensely colored chromogen. Condensed product of pregabalin at its λmax 461 nm shows linearity in the concentration range of 50-450 μg/ml. The relative standard deviations were 0.487% and 0.25% respectively for 2 methods. Linear relationships with good correlation coefficients (0.993-0.998) were found between absorbance and the corresponding concentrations of the drug. The reliability and performance of the proposed methods was validated statistically. Percentage recovery ranged from 100.5 and 99.867 respectively.