Fungal Genetics Reports · 1990 · 53 citations · 0 references
Life SciencesMicrobiologyMolecular BiologyMicrobial GeneticsMolecular BiotechnologyMycologyFungal BiotechnologyNucleic Acid PurificationNucleic Acid SynthesisProtoplast CultureRestriction Enzyme TechnologyDigestionDouble-stranded Dna Library PreparationLow-input Dna Library PreparationProtoplast TechnologyRestriction Enzyme DigestionRestriction Enzyme Purification
Most methods of DNA preparation from fungi are time-consuming due to the need to first make protoplasts, expensive for chemicals such as cesium chloride, or suitable only for small scale preparations. We have developed a simple method for total DNA preparation, yielding a product of quality suitable for restriction digestion and library construction.