Cellular Physiology and Biochemistry · 2017 · 52 citations · 21 references
Life SciencesImmunologyPharmacologyCell SignalingInflammationInnate ImmunityAnti-inflammatory PharmacologyCytokine BiologyInflammatory Cell ResponsesInflammatory MediatorsInflammatory SignalingMacrophage BiologyAnti-inflammatory TherapeuticsAssayCytokine-mediated Cell DeathEndotoxin-mediated InflammationMacrophage-mediated InflammationProinflammatory Cytokine ModulationProinflammatory Mediator ReleaseProinflammatory Mediators
BACKGROUND/AIMS: Endotoxemia is a life-threatening situation that signifies a key challenge in the field of intensive care medicine. Proinflammatory mediators produced by macrophages play a key role in endotoxemia. Gelsolin (GSN) is involved in the process of inflammation. METHODS: IL-6 and TNF-α in the supernatant were measured with an ELISA kit. NO production was assessed by measurement of nitrite concentration with the Griess assay. si-RNA directed against GSN (si-GSN) was transfected by Lipofectamine. RESULTS: LPS decreased the levels of GSN. Recombinant GSN inhibited the cytokines induced by LPS and rescued mice from LPS-induced death, and si-GSN increased death in the LPS-pretreated mice. CONCLUSION: GSN exhibited a protective role in endotoxemia.
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Endotoxemia in Human Septic Shock
Robert L. Danner, Ronald J. Elin, J M Hosseini et al. · CHEST Journal · 1991 · 666 citations