Hplc–MS/MS Method for Dexmedetomidine Quantification with Design of Experiments Approach: Application to Pediatric Pharmacokinetic Study

Abstract

Application of Design of Experiments approach allowed for fast and efficient analytical method development and validation as well as for reduced usage of chemicals necessary for regular method optimization. The proposed technique was applied to determination of DEX pharmacokinetics in pediatric patients undergoing long-term sedation in the intensive care unit.