Improving of Rice Blast Resistances in Japonica by Pyramiding Major R Genes

Abstract

Rice blast, caused by the fungal pathogen <i>Magnaporthe oryzae</i>, is a major constraint to rice production worldwide. In this study, we developed monogenic near-isogenic lines (NILs) NIL ^<i>Pi</i>9, NIL <i>^Pizt</i> , and NIL ^<i>Pi</i>54 carrying genes <i>Pi9</i>, <i>Pizt</i>, and <i>Pi54</i>, respectively, by marker assisted backcross breeding using 07GY31 as the <i>japonica</i> genetic background with good agronomic traits. Polygene pyramid lines (PPLs) PPL ^{<i>Pi</i>9+<i>Pi</i>54} combining <i>Pi9</i> with <i>Pi54</i>, and PPL ^{<i>Pizt</i>+<i>Pi</i>54} combining <i>Pizt</i> with <i>Pi54</i> were then developed using corresponding NILs with genetic background recovery rates of more than 97%. Compared to 07GY31, the above NILs and PPLs exhibited significantly enhanced resistance frequencies (RFs) for both leaf and panicle blasts. RFs of both PPLs for leaf blast were somewhat higher than those of their own parental NILs, respectively, and PPL <i>^Pizt</i>⁺^<i>Pi</i>54 exhibited higher RF for panicle blast than NIL <i>^Pizt</i> and NIL ^<i>Pi</i>54 (<i>P</i> < 0.001), hinting an additive effect on the resistance. However, PPL ^{<i>Pi</i>9+<i>Pi</i>54} exhibited lower RF for panicle blast than NIL ^<i>Pi</i>9 (<i>P</i> < 0.001), failing to realize an additive effect. PPL <i>^Pizt</i>⁺^<i>Pi</i>54 showed higher resistant level for panicle blast and better additive effects on the resistance than PPL ^{<i>Pi</i>9+<i>Pi</i>54}. It was suggested that major R genes interacted with each other in a way more complex than additive effect in determining panicle blast resistance levels. Genotyping by sequencing analysis and extreme-phenotype genome-wide association study further confirmed the above results. Moreover, data showed that pyramiding multiple resistance genes did not affect the performance of basic agronomic traits. So the way to enhance levels of leaf and panicle blast resistances for rice breeding in this study is effective and may serve as a reference for breeders. <b>Key Message:</b> Resistant levels of rice blast is resulted from different combinations of major R genes, PPL <i>^Pizt</i>⁺^<i>Pi</i>54 showed higher resistant level and better additive effects on the panicle blast resistance than PPL ^{<i>Pi</i>9+<i>Pi</i>54}.