Contribution of hydrogen peroxide to non‐thermal atmospheric pressure plasma induced A549 lung cancer cell damage

Abstract

Non‐thermal plasma (NTP) can induce cancer cell death which is supposed to be mediated by oxidative stress. In this work, the contribution of hydrogen peroxide (H 2 O 2 ) in NTP induced lung cancer cell damage is quantitatively studied. Our results also show signification production of H 2 O 2 in plasma activated medium (PAM). NTP treatment induce critical raising of cell death, apoptosis, and DNA double‐strand breaks (DSBs) in A549 cell. NTP treatment also cause mitochondrial dysfunction and intracellular ROS production in A549 cells. Furthermore, catalase efficiently protect cells from NTP induced A549 cell damage. This work clarified the contribution of H 2 O 2 to plasma induced cancer cell damage.