Sub-ppb Level Detection of 2-Hydroxybiphenyl Using Portable Surface-Plasmon-Resonance Immunosensor

Abstract

A sensor-chip-module integrated portable surface-plasmon-resonance (SPR) immunosensor was employed for monitoring the indirect competitive immunoreaction of the monoclonal antibody against 2-hydroxybiphenyl between the analyte (2-hydroxybiphenyl, abbreviated as HBP hereafter) in test solution and the HBP-protein conjugate immobilized on sensor chip. This sensor was found to detect HBP rather sensitively in the concentration range of 0.1-1000 ppb. The sensor chip could be reusable several times by removing the antibody anchored to the sensor chip on treatment with a pepsin solution (pH 2.0) for a few minutes.