Effect of Some Levels of Cardamom, Clove and Anise on Hepatotoxicity in Rats Caused by CCL 4

Abstract

The aim of this study was to investigate the effect of three levels of cardamom, clove and anise on rats suffering from hepatotoxicity. Fifty five male albino rats (Sprague Dawley Strain) used in this study, the rats divided into eleven groups. The first and second groups fed on basal diet as a control negative and positive groups. The third, fourth and fifth groups were fed on basal diet containing (2%, 4% and 6% cardamom, respectively), the sixth, seventh and eighth groups were fed on basal diet containing (2%, 4% and 6% clove, respectively), the ninth, tenth and eleventh groups were fed on basal diet containing (2%, 4% and 6% anise, respectively). On the 27 day of the experiment, all animals, except the negative control group was administered subcutaneous injection 20% CCl in liquid paraffin oil at a dose 5 ml/kg body weight to induce 4 hepatotoxicity in rats. During the experimental period (28 days), the diets consumed and body weights were recorded twice weekly, at the end of the experiment period, the rats were fasted overnight, then the rats were anaesthetized and sacrificed and the blood samples were collected from the aorta. The blood samples were centrifuged and serum was separated to determine some biochemical parameters. Results showed that, feeding rats on basal diet and injected with CCl (control positive group) induced non-significant changes in 4 fed intake and body weight gain %, while liver and kidney weights, liver enzymes, lipid profile, except HDL-c, glucose and kidney functions increased significantly increase p?0.5, as compared to the rats fed on basal diet in the first main group (control negative group). Treating rats which suffer from hepatotoxicity with the three levels from (cardamom, clove and anise) decreased body weight gain%, liver and kidney weights/body weight%, serum AST, ALT, ALP, cholesterol, triglycerides, LDL-c, VLDL-c, uric acid, urea nitrogen, creatinine, glucose, while HDL-c increased. The highest improvement in these parameters recorded for the group which treated with the high level of clove (6%). In conclusion, the results of this study demonstrate that clove, cardamom and anise increased antioxidant activity and improved hepatotoxicity of rats.