Diversity-Oriented Enzymatic Modular Assembly of ABO Histo-blood Group Antigens

Abstract

Enzymatic synthesis of all 15 naturally occurring human ABH antigens was achieved using a diversity-oriented enzymatic modular assembly (EMA) strategy. Three enzyme modules were developed, each one-pot multienzyme module comprises a glycosyltransferase and one or two corresponding sugar nucleotide generating enzyme(s). These multienzyme cascade processes provide an efficient and convenient platform for collective synthesis of all 15 ABH antigens in three operationally simple steps from five readily available disaccharide acceptors and three simple free sugars as donor precursors.