Screenings of In-vitro antimicrobial, cytotoxic and anti-inflammatory activity of crude methanolic extracts of Crinum latifolium (Leaves)

Abstract

Crude methanolic extracts of Crinum latifolium plant (Leaves) was assayed to identify various pharmacological properties. Antimicrobial potential of crude methanolic extracts of C. latifolium was accomplished by most commonly used disc diffusion method against a wide range of Gram positive (+ve) and Gram negative(-ve) bacteria. Extracts showed slight antimicrobial activity against Gram positive (+ve) bacteria while surprisingly showed significant antimicrobial activity against Gram negative (-ve) bacteria Escherichia coli. In contrast to vincristine sulphate, the crude methanolic, n-hexane soluble, petroleum ether soluble and chloroform soluble extracts showed slight to moderate cytotoxic properties with LC50 value of 7.06, 48.978, 242.83 and 153.93 µg/ml respectively. Plant extract showed significant (P<0.05) anti-inflammatory properties, that is, 16.21 and 20.55%10 mg/ml for hypotonic solution and heat induced condition respectively. So, this plant extract demands further research for revealing all its potency to have new safe drug for the entire respective field of medical science. Key words: Crinum latifolium, zone of inhibition, brine shrimp lethality bioassay, anti-inflammatory.