Regulation of proline biosynthesis, degradation, uptake and transport in higher plants: Its implications in plant growth and abiotic stress tolerance

TLDR

Proline accumulates in plants under salt, drought, and metal stress, yet its role in development and stress recovery remains poorly understood. This review summarizes the genes that regulate proline biosynthesis, degradation, and uptake. It details transgenic strategies for proline overproduction, associated metabolic pathways, and emerging signal‑transduction mechanisms.

Abstract

Dramatic accumulation of proline due to increased synthesis and decreased degradation under a variety of stress conditions such as salt, drought and metal has been documented in many plants. Similarly, a decrease in the level of accumulated proline in the rehydrated plants is due to both down regulation of proline biosynthetic pathway enzymes and upregulation of proline degrading enzymes. But, the role of proline during plant development and the molecular basis of the effect of proline accumulation during stress and upon relief of stress are still largely obscure. Here, we summarize the genes governing the proline biosynthetic pathway, its degradation and regulation. Sequentially, we provide an account on transgenics raised so far to engineer the overproduction of osmolyte proline. Also, the identification of specific cellular pathways involved in proline biosynthesis and metabolic changes occurring in transgenic plants developed for proline enhancements are discussed. Further, emphasis is also made on an untouched area of signal transduction of proline biosynthetic pathway.