The Number, Organization, and Size of Polymorphic Membrane Protein Coding Sequences<b> </b>as well as the Most Conserved Pmp Protein Differ within and across <b><i>Chlamydia</i></b> Species

Abstract

Variation is a central trait of the polymorphic membrane protein (Pmp) family. The number of <i>pmp</i> coding sequences differs between <i>Chlamydia</i> species, but it is unknown whether the number of <i>pmp</i> coding sequences is constant within a <i>Chlamydia</i> species. The level of conservation of the Pmp proteins has previously only been determined for <i>Chlamydia trachomatis.</i> As different Pmp proteins might be indispensible for the pathogenesis of different <i>Chlamydia </i>species, this study investigated the conservation of Pmp proteins both within and across <i>C. trachomatis,</i><i>C. pneumoniae,</i><i>C. abortus,</i> and <i>C. psittaci.</i> The <i>pmp</i> coding sequences were annotated in 16 <i>C. trachomatis,</i> 6 <i>C. pneumoniae,</i> 2 <i>C. abortus,</i> and 16 <i>C. psittaci</i> genomes. The number and organization of polymorphic membrane coding sequences differed within and across the analyzed <i>Chlamydia </i>species. The length of coding sequences of <i>pmpA,</i><i>pmpB,</i> and <i>pmpH</i> was conserved among all analyzed genomes, while the length of <i>pmpE/F</i> and <i>pmpG,</i> and remarkably also of the subtype <i>pmpD,</i> differed among the analyzed genomes. PmpD, PmpA, PmpH, and PmpA were the most conserved Pmp in <i>C. trachomatis,</i><i>C. pneumoniae,</i><i>C. abortus,</i> and <i>C. psittaci</i>, respectively. PmpB was the most conserved Pmp across the 4 analyzed <i>Chlamydia</i> species.