Using Raman spectroscopy to estimate the demineralization of bone transplants during preparation

Abstract

This paper presents the results of experimental studies of samples of human cortical and spongy bone tissue by means of Raman scattering as a function of the degree of demineralization. Raman spectra are obtained for samples of human cortical and spongy bone tissue as a function of the degree of demineralization. The dependence of the intensity ratio on the demineralization time, which characterizes the degree of demineralization, is studied for the Raman peaks at 950–962 (PO4)3− and 1665 cm−1 (amide I).