Matrix Solid-Phase Dispersion Isolation and Liquid Chromatographic Determination of Oxolinic Acid in Channel Catfish (Ictalurus punctatus) Muscle Tissue

Abstract

Abstract Matrix solid-phase dispersion Isolation and liquid chromatographic techniques were developed to quantify oxolinic acid (OA) and OA-related metabolites in channel catfish (Ictalurus punctatus) muscle tissue and bile. Mean percent recovery, correlation coefficient, and inter- and intra-assay variabilities were 82.8 ±15.0%, 0.996 ±0.004,12.5 ±8.9%, and 1.22%, respectively, for OA Isolated from fortified muscle tissue. Using the methodologies described in the current study, incurred OA In muscle tissue and 4 OA-related metabolites were Isolated In the bile of dosed catfish.