Integrated Multi-sensor System for Parallel In-situ Monitoring of Cell Nutrients, Metabolites and Cell Mass in Biotechnological Processes

Abstract

We report on a multi-sensor system for the parallel in-situ monitoring of cell nutrients, metabolites and cell mass in biotechnological processes. The integration of enzyme sensors and impedance spectroscopy on one chip for applications in bioreactors is demonstrated for the first time to our best knowledge. Measurements of glucose and lactate concentrations in a wide range are shown with linear ranges up to 600 mM and 900 mM, respectively. The glucose and cell mass sensor in parallel are successfully employed in a cell culture of Saccharomyces cerevisiae to monitor glucose consumption and increasing mass of the cells.