Effect of genotype on micropropagation of walnut trees (Juglans regia)

Abstract

Embryos of Juglans regia originating from elite trees, selected for their wood quality were cultured on DKW medium supplemented with 4.44 μM 6-benzylaminopurine (BA) and 0.005 μM indole-3-butyric acid (IBA). Results on the effects of cytokinin (BA) and auxin (IBA) on shoot development indicated that elongation is enhanced with 2.22 μM BA while the formation of new axillary shoots is favored by 4.44 μM BA and 0.005 μM IBA. It was, also, observed that different genotypes had different requirements for growth regulators. Significant differences were observed among the multiplication rates of twelve different clones of J. regia. The effect of genotype is obvious in the rooting phase, as well. Some clones exhibit high rooting ability (95 %) and some low (5 %).