Biochemical studies on 2-deoxy-scyllo-inosose, an early intermediate in the biosynthesis of 2-deoxystreptamine. III. Confirmation of in vitro synthesis of 2-deoxy-scyllo-inosose, the earliest intermediate in the biosynthesis of 2-deoxystreptamine, using cell free preparations of Streptomyces fradiae.

Abstract

The in vitro activity of formation of 2-deoxy-scyllo-inosose, the earliest intermediate of the 2-deoxystreptamine biosynthesis, was confirmed in the precipitate fraction obtained by 30-45% saturation of ammonium sulfate from the 15, 000×g supernatant of the sonicated Streptomyces fradiae IFO 13147 cells.