Using Microfluidics for Fast, Accurate Measurement of Lysozyme Nucleation Kinetics

Abstract

Here we measure lysozyme nucleation kinetics using an easy-to-use and simply constructed microfluidics setup previously described. We confirm that microfluidics is a direct, accurate, and fast method to measure nucleation frequency using only a few milligrams of molecules. Moreover, our microfluidics setup, by diminishing crystallizer volumes, increases the experimental supersaturation range accessible and can be applied to all water-soluble molecules.