Embryogenesis and plant regeneration from mesocarp of Psidium guajava L. (guava)

Abstract

A protocol has been developed for the induction, maturation and germination of somatic embryos from mesocarp tissue of Psidium guava L. (guava). Explants were cultured in modified MS medium fortified with 2, 4-D (2.0 mg/l), ascorbic acid (100 mg/l), L-glutamine (400 mg/l) and sucrose (6%). Embryogenic proliferating tissue was induced, which was translucent, mucilaginous and differentiated into many small somatic embryos. The somatic embryos were retained in the same medium, where simultaneously differentiation of new somatic embryos and their conversion into plantlets was observed. Thus, the embryogenesis in guava could be perpetuated, which would be used in future for carrying out cellular selection against wilt causing organism.