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An improved method for the detection of peptide-induced upregulation of HLA-A2 molecules on TAP-deficient T2 cells.
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1996
Year
Improved MethodMhc ClassT-regulatory CellHla ImmunogeneticsImmunologyImmunologic MechanismAntigen ProcessingPeptide SciencePeptide BindingT2 AssayImmunotherapyTap-deficient T2 CellsCell SignalingAllergyAutoimmunityT Cell ImmunityCell BiologySignal TransductionHla-a2 MoleculesHla TypingCellular Immune ResponseMedicine
Peptide-induced upregulation of MHC class I on TAP-deficient T2 cells is used to monitor peptide binding to class I molecules. We present a temperature-dependent modification of the T2 assay which increases the sensitivity of peptide binding to MHC class I with several orders of magnitude.