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Post-capillary Immobilon-P membrane fraction collection for capillary electrophoresis.
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1993
Year
EngineeringProtein PurificationSeparation ScienceBioanalysisAnalytical ChemistryClinical ChemistryMicrofluidicsFraction Collection ProcessIsotachophoresisBiophysicsChromatographyCapillary ElectrophoresisBiochemistryBiomedical AnalysisMembrane PermeationAnalyte DilutionContinuous Fraction CollectionField-flow FractionationMedicine
A simple, cost-effective and efficient method of continuous fraction collection for capillary electrophoresis is described. The method employs a membrane, such as polyvinylidene difluoride, onto which the separated components from a capillary electrophoresis capillary are directly collected to overcome the problem of analyte dilution and to facilitate the fraction collection process. Model proteins are separated and collected onto a polyvinylidene difluoride membrane.