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Transformation of sitosterol to androsta-1, 4-diene-3, 17-dione by immobilized Mycobacterium cells.
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1991
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EngineeringBacteriologyMolecular BiologyEnzyme ImmobilizationBiosynthesisBiochemical EngineeringMycobacterium CellsNatural Product BiosynthesisPolymer ChemistryBiotransformationNatural PolymerVarious Polymeric MatricesMolecular MicrobiologyImmobilized Mycobacterium CellsBiomolecular EngineeringBiomanufacturingBiotechnologySynthetic BiologyImmobilized EnzymeMicrobiologyMedicineOptimum Catalytic Stability
Transformation of sitosterol to androsta-1, 4-diene-3, 17-dione was studied with Mycobacterium cells entrapped in various polymeric matrices. Of the three supports viz. alginate, carageenan, agarose and polyacrylamide, studied, the polyacrylamide immobilized cells showed optimum catalytic stability and reusability.