Induction of Organogenesis in Callus Cultures of Nigella sativa L.

Abstract

The present work examines the role of auxins and kinetin in inducing organogenesis in callus cultures of Nigella sativa L. Differentiation of roots and shoots occurred after removing auxin and kinetin from the medium. But the presence of auxin for a certain period is essential before putting the callus into auxin-free medium Reversal of callus to roots, and of roots to callus was repeated several times by transferring the inoculum from hormone-rich medium to hormone-free medium and vice versa Cytological studies of the roots and leaf-tips of differentiated plants revealed that in N sativa organogenesis occurs only from diploid cells