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Analysis of the TNF receptor on KYM cells by binding assay and affinity cross-linking.
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1988
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ImmunologyImmunoeditingCell DeathPathologyImmunologic MechanismCancer BiologyCellular PhysiologyTumor BiologyInflammationTumor Necrosis FactorSignaling PathwayCell SignalingAffinity Cross-linkingCell BiologyMalignant DiseaseTumor MicroenvironmentCytokineSignal TransductionKym CellsTnf ReceptorCross-linking AnalysisCellular BiochemistryMedicine
The existence of a tumor necrosis factor (TNF) receptor on KYM human myosarcoma cells was elucidated by Scatchard plot and cross-linking analysis. The average number of specific binding sites per cell was 15,300 and the apparent dissociation constant Kd was 4.0 X 10(-10) M. The estimated relative molecular mass of a TNF receptor on KYM cell was approximately 95,000.