Quantitative microanalysis of bile.

Abstract

Abstract A quantitative microanalytical procedure for bile has been developed which is based on the solvent partition of bile constituents into subgroups followed by silicic acid column chromatography, gas chromatography, and thin-layer chromatography. This method can be performed with less than 1 ml. of bile and can easily be scaled down to be applicable for experimentation on smaller animals. This method gives information on a wide variety of constituents present in bile such as total phospholipids; lecithin; and its fatty acids composition; cholic, chenodeoxycholic, deoxyoholic, and lithocholic acids; free fatty acids; cholesterol; mono-, di-, and triglycerides; bilirubin; total solid; and total lipids. Quantitative analysis of human gallbladder bile has been presented.