Biosynthesis of Iron oxide nanoparticles and its haematological effects on fresh water fish Oreochromis mossambicus

Abstract

Iron oxide nanoparticles were synthesized biologically using potato as starch template (FeSo 4.7H 2O as substrate). The X-ray diffraction (XRD) and Scanning Electron Microscope (SEM) analysis revealed that the particles were spherical in shape and the size ranges from 29-40 nm. Toxicity tests were performed to investigate the possible harmful effects on fresh water fish Oreochromis mossambicus exposed to as synthesized α -Fe 2O3 NPs at exponential concentrations (0.5, 5 and 50 μg/mL). The significant changes in haematological (RBC, WBC, Hb, HCT) and biochemical parameters (SGOT, SGPT) were observed after 96 h in treated groups than control, which may be a consequence of iron oxide NPs in the blood cells of fish.