Microprobe analysis of maturation-related elemental changes in rat parotid secretory granules

Abstract

Electron probe X-ray microanalysis was use to quantitate the elemental and mass changes that take place during the secretory granule maturation process. A single injection of isoproterenol stimulated the depletion of secretory granules from rat parotid acinar cells. Granules at different stages of maturation were analyzed as they reaccumulated within the cells over time. Dry mass measurements revealed that secretory material becomes concentrated about twofold within maturing granules. Nearly all of the increase in mass concentration could be attributed to a reduction in water space. Data are presented that indicate that Na, K, Cl, and water all efflux from secretory granules during maturation. In contrast, granule S content is positively correlated with maturation. Hence, significant changes in granule elemental and water contents occur during the maturation process.