Detection of A RAPD Marker Linked to Dominant Multi-ovary Gene in Wheat (Triticum aestivum)

Abstract

Using BSA method, a dominant multi-ovary line of common wheat (Triticum aestivum) was crossed with variety Xinong 1376(T. aestivum). From F2 segregation population, a multi-ovary and a single-ovary gene pools were established; via RAPD-PCR method, single-ovary and multi-ovary mixed populations were screened using 440 random primers. The result indicated that the expanded product of 10 primers showed specific character among neaisogenic lines, multi-ovary population and its parent had band, while single-ovary population and its parent didn't. The expanded product of 6 primers showed the opposite character that the product of single-ovary line had band and that of multi-ovary line had not. After repeating tests and checking up individual plant, only primer S236 showed the stable multi-morphological character to multi-ovary parent, mixed population and single plant, so it could be used as a molecule marker of the dominant multi- ovary gene.