Isolation and Identification of Lipopeptides and Volatile Compounds Produced by Bacillus subtilis Strain BAB-1

Abstract

【Objective】 The objective of this study was to isolate and identify the lipopeptides and volatile compounds produced by Bacillus subtilis strain BAB-1,and to analyze their antifungal activities.【Method】 Lipopepetide compounds were isolated,purified and identified by using HPLC and MALDI-TOF-MS techniques,and the volatile compounds(VCs) were identified by using GC-MS method.【Result】 Three fractions of the lipopeptides,LP-1,LP-2 and LP-3,were collected,among which LP-2 showed the major antifungal activity.According to the mass spectra analysis,LP-1 was determined to be identical to surfactin,varying in the length of the fatty acid chain moiety from C13 to C15.LP-2 consisted of fengycin A(C14—C15) and fengycin B(C14—C18),and LP-3 remained undetermined.Many different kinds of VCs were identified,such as alcohols,ketones,acids,amines,alkanes and so on.【Conclusion】 B.subtilis strain BAB-1 can produce lipopeptides and VCs both with strong antifungal activities.