High Sensitive Voltammetric Levothyroxine Sodium Determination on Renewable Mercury Film Silver Based Electrode

Abstract

Levothyroxine sodium (LS, L-T4) is a sodium salt of natural thyroid hormone thyroxine (T4), commonly used in treatment of hypothyroidism. In this work a new voltammetric method of levothyroxine sodium (LS) determination using renewable mercury film electrode is presented. A linear voltammetric response was obtained for analyte in the concentration range from 0.025·10−6 mol L−1 (1.99·10−5 g L−1) to 4.0·10−6 mol L−1 (3.19·10−3 g L−1), with a detection limit of 1.8·10−8 mol L−1 for preconcentration time of 30 s. Repeatability of method was determined as RSD % for levothyroxine sodium concentration of 0.2·10−6 mol L−1 as 1.4% (n = 9). The proposed method was successfully applied and validated by studying the recovery of levothyroxine sodium in different pharmaceutical products.