In vitro selection and evolution of functional proteins by using ribosome display

Abstract

Ribosome display technology,which developed in the middle of1990,becomes a simple and efficient tool for in vitro selection and evolution of functional molecules.Ribosome display is also the first method for screen-ing and selection of functional proteins performed completely in vitro and circumventing many drawbacks of in vivo systems.Here the author reviews the principle of ribosome display and its strategy and characteristics in applica-tions,and follows the update advances and development of the pioneering in ribosome display technology.