The Effects of Arolycoricidine and Narciprimine on Tumor Cell Killing and Topoisomerase Activity

Abstract

In this study, narciprimine and arolycoricidine wer e isolated from G. rizehensis Stern (Amaryllidaceae). The structures of the alkaloids w ere elucidated by spectroscopic methods (1D NMR, EI-MS). Due to the previous reports on anti-cancer activity of this group of alkaloids, we investigated their effects on DNA topoisomerase reactions, which are known as the cellular targets of a number of chemotherapeutical drugs. The results revealed that arolycoricidine and narci primine were effective in both type I and type II D NA topoisomerase reactions in a dose-dependent manner. Topoisomerase-interfering ability of these alkaloi ds partially correlated with cytostaticity assays, usi ng HeLa (cervix adenocarcinoma), MCF7 (breast adenocarcinoma) and A431 (skin epidermoid carcinoma) cells. Our results are discussed in relation to t he potential significance of these alkaloids in the co urse of drug-development studies.