BIOGENIC SILVER NANOPARTICLES FROM SPINACIA OLERACEA AND LACTUCA SATIVA AND THEIR POTENTIAL ANTIMICROBIAL ACTIVITY

Abstract

Development of biologically inspired experimental processes for the synthesis of nanoparticles is an important branch of nanotechnology and is expected to open new avenue to fight and prevent disease. Many researches and studies are evolving regarding the green synthesis of nanoparticles due to the challenges faced by chemical synthesis protocol of nanoparticles in being toxic, flammable and unstable. In this study we report the synthesis of silver nanoparticles from Spinacia oleracea and Lactuca sativa leaves by ‘exploiting’ the reduction capabilities of varied phytochemicals present in it, as confirmed by the FTIR characterization analysis technique. UV-Vis Spectroscopy interpreted the formation of silver nanoparticles by the reduction of silver salts, respectively. The biogenic nanoparticles synthesized using lettuce and spinach leaves exhibited variety of shapes, exposed by SEM and TEM. Due to increasing development of the resistance to the existing antibiotic and various drugs, we extended our research to utilize the efficacy of these synthesized nanoparticles as antimicrobial agents, against B.subtilis, S.aureus, K.pneumonia and E. faecalis strains. Our results specify that the bactericidal properties of the nanoparticles present a direct interaction with the bacterias.