Characterization of an acidic tripeptide in neurotoxic dialysate.

Abstract

An acidic tripeptide was isolated from neurotoxic dialysate by ultrafiltration with an Amicon Centriflo DM-5 membrane, followed by gel filtration on Sephadex G-15 and Sephadex G-10, and ion-exchange chromatography on DEAE-Sephadex A-25. The tripeptide thus obtained was identified as H-Glu-Asp-Gly-OH by amino acid analysis, Edman degradation, and measurement of physical constants and analytical data in comparison with those of the authentic tripeptide. This tripeptide inhibited LDH activity.