Preparation of Water-Soluble Combination Products of Gossypol and their Toxicity to Aquarium Fish.

Abstract

(1) Gossypol was combined with proteins, amino acids, and carbohydrate materials by a procedure involving mixing of the gossypol and other combining substances in alkaline solutions and subsequent lyophilization of the neutralized frozen solutions. (2) Crystalline gossypol when added to the aquarium bath had no visible effect upon goldfish. The water-soluble combination products of gossypol were toxic to fish in concentrations as small as one part of gossypol product to a hundred thousand parts of water. (3) Separated pigment glands were toxic to fish but were significantly less toxic than the water-soluble combination products of gossypol.