American Journal of Clinical Pathology · 1983 · 94 citations · 0 references
MedicineDermatologyPathologyAnatomic PathologyDermatopathologyHistopathologyCutaneous NeoplasmsHistiocytic DisordersImmunopathology Of SkinInflammatory DermatosesInflammatory Skin DiseasesInflammatory Skin DisordersCutaneous HistiocytosisDermal Inflammatory InfiltratesDermatopathologic Lesion DescriptionHistopathology Of The SkinImmune InfiltratesIn Situ CharacterizationInterstitial Histiocytic InfiltratesPerivascular Lymphocytic Infiltrate
Cutaneous lesions in three cases of histiocytosis-X were studied by light microscopy, electron microscopy, and immunoperoxidase technics. In each case, Birbeck granule-containing histiocytosis-X cells infiltrated the epidermis and were apposed to lymphocytes. The histiocytosis-X cells and normal Langerhans cells stained with anti-T6 and anti-I1 (Ia-like) antibodies but were negative with anti-T3, anti-T8, anti-M1, and anti-lysozyme antibodies. In addition, the histiocytosis-X cells also stained with anti-T4 antibodies, which react with T-cells associated with helper/inducer phenotype. This study supports the hypothesis that histiocytosis-X cells are abnormal Langerhans cells. The presence of T4/T6-positive cells in cutaneous disease may be a marker for abnormal Langerhans cells.