Arzneimittelforschung · 2011 · 21 citations · 0 references
AgricultureNutritional SciencesOncologyCellular MetabolismHuman NutritionMicronutrient NutritionAmino Acid MetabolismAnimal Cell CultureCancer MetabolismMammalian Cell CultureNutrition During Cancer CareOncology NutritionCell Growth RegulationMethionine MetabolismOncology Nutrition TherapyParenteral Amino Acid NutritionSynthetic Amino Acid SupplementationTotal Amino Acid ContentTotal Parenteral Nutrition SolutionsTumor Growth Rate
The effects of a nutrition therapy with D-methionine (Met)-containing solution were investigated in cell cultures of the AH109A cell line. The growth of AH109A hepatoma cells in culture media with D-Met-supplemented medium, L-Met-supplemented medium (control) and Met-free medium was compared. The D-Met-supplemented medium inhibited the cell growth to an extent similar to that manifested in the Met-free medium. The total free amino acid concentrations in the control medium decreased by approximately 40% on day 6 post-culture. However, the free amino acid concentrations in D-Met-supplemented and Met-free media did not change. Furthermore, alanine, which was not added to RPMI-1640, was detected in the control medium on day 6 post-culture. These results suggest the possibility of application of D-Met-containing solution to cancer patients receiving total parenteral nutrition.