Agricultural and Biological Chemistry · 1989 · 16 citations · 0 references
Life SciencesGeneticsMolecular BiologyGene ExpressionTranscriptionTranscriptional RegulationTranscription In EukaryotesTranscription In ProkaryotesTranscription InitiationTranscription Start SitesTranscriptional Regulation In EukaryotesYeast GeneticsTata Box RecognitionTata Box-dependent InitiationTranscriptional Start SitesUpstream Promoter ElementsUpstream Sequence ElementsYeast Gene Expression RegulationYeast Gene FunctionYeast Gene Regulation
Deletion analysis was used to identify sequences upstream of the STA1 gene of Sacchavomyces diastaticus that are required for its expression. Our analysis revealed that STA1 has at least three upstream activation sequences (UAS): UAS1 and UAS2-2 were required for full transcriptional activity, while deletions removing UAS2-1 were still transcribed but the transcripts failed to be translated. These sequences contained both A + T-rich and inverted repeat sequences that might serve as components of the upstream activation sites. We also showed that either deletion of a TATA homologue or insertion of palindromic DNA within the region between the TATA box and the mRNA start sites had no effect on the level of STA1 transcription but the transcripts failed to initiate properly.